Anti-CD32 (Fc Gamma RIIa)(FCGR2A/479), Biotin conjugate

• Description:

  This MAb binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i. e. , IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD32A; Fc fragment of IgG low affinity IIa receptor; Fc gamma RIIa; FCGR2A; FcgRII; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FCGR2A

• Gene ID:

  2212

• NCBI Gene ID:

  352642

• UniProt:

  P12318

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human FCGR2A protein

• Target Antigen:

  CD32 | Fc Gamma RIIa

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  FCGR2A/479

• Conjugation:

  Biotin

• Disease:

  Tumor

• Source:

  Animal

• Positive Control:

  K-562 cells. Lymph node, tonsil and placenta.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  40 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4