Anti-Hu CD118 Purified

Anti-Hu CD118 Purified

• Background: CD118 (LIFR alpha) is a type I transmembrane glycoprotein of the cytokine receptor family, which associates noncovalently with CD130 to form the functional high affinity LIF receptor, which also acts as an oncostatin M receptor. CD118 alone binds LIF with low affinity. A secreted form of CD118 results from alternative splicing, and may have inhibitory effect, as it also binds LIF, although with low affinity. CD118 is not expressed on lymphocytes, but it is widely expressed outside the immune system. Soluble CD118 level rises during pregnancy, in parallel with a drop in circulating LIF levels.
• Specifications: The mouse monoclonal antibody 12D3 recognizes an extracellular epitope of CD118, a transmembrane glycoprotein, which associates with CD130 to form the functional high affinity LIF receptor.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: human recombinant soluble CD118-Fc
• Target Antigen: CD118
• Isotype: Igg1 κ
• Clone: 12D3
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC, WB, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Taupin JL, Legembre P, Bitard J, Daburon S, Pitard V, Blanchard F, Duplomb L, Godard A, Jacques Y, Moreau JF: Identification of agonistic and antagonistic antibodies against gp190, the leukemia inhibitory factor receptor, reveals distinct roles for its two cytokine-binding domains. J Biol Chem. 2001 Dec 21276(51):47975-81., URL: https://pubmed.ncbi.nlm.nih.gov/11606572/,
• Storage Conditions: Store at 2-8°C. Do not freeze.