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Tilmicosin ELISA Kit

CAT: 0026-E4943-100Size: 96 AssaysDry Ice: NoHazardous: No
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CAT#:0026-E4943-100Size:96 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tilmicosin is a semi-synthetic macrolide antibiotic that is structurally similar to Tylosin. As a macrolide, its main function is to bind to 23s rRNA of the 50S ribosomal subunit of the bacteria. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. It targets both gram-positive and gram-negative bacteria that cause respiratory infection in cattle and pigs. BioVision’s Tilmicosin ELISA kit is used to quantitatively measure Tilmicosin in muscle and liver tissue, honey, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Tilmicosin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Tilmicosin in the samples.
CAS Number
7732-18-5
Reactivity
Universal
Applications
Competitive ELISA kit to quantitatively measure Tilmicosin in tissue, honey, milk, and egg samples
Detection Method
Absorbance (450 nm)
Features & Benefits
This ELISA kit is used for in vitro quantitative determination of Tilmicosin 0.5ppb for muscle (Method 1), 10ppb for muscle (Method 2), 10ppb for liver, 1ppb for honey, 5ppb for milk and eggs Cross reaction: Tilmicosin 100%, Tylosin 1% Sensitivity: 0.5ppb
Shipping Conditions
Gel Pack
Storage Conditions
4ºC
Shelf Life
12 months

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