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NSE Antibody

CAT: 0013-GTR18317880Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18317880Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
UniProt
P09104
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the NSE antibody.
Clonality
Monoclonal
Clone
ENO2/7447
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the NSE antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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