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Recombinant SMAD4 Antibody

CAT: 0013-GTR18317225Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18317225Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-beta family phosphorylate SMAD proteins (SMAD1 and SMAD2) . These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%) . SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.
UniProt
Q13485
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 400-552) from the human protein was used as the immunogen for the recombinant SMAD4 antibody.
Clonality
Recombinant
Clone
SMAD4/7906R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant SMAD4 antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa

UniProtKB · Q13485

SMAD family member 4

SMAD4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13485
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMAD4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMAD 4, hSMAD4
EC number
—
Processing
—
Secondary accessions
A8K405
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

CytoplasmNucleus

Disease

Disease variant

Molecular function

DNA-binding

Ligand

Metal-bindingZinc

Biological process

TranscriptionTranscription regulation

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