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HDEL Antibody

CAT: 0013-GTR18316536Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18316536Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Anti-Yeast HDEL Monoclonal IgG2b
Product Name Alternative
H-D-E-L (his-asp-glu-leu), endoplasmic reticulum, luminal ER protein retention, KDELR1, Endoplasmic reticulum retention signal
Reactivity
Drosophila, Plant, Saccharomyces, Yeast
Immunogen
Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Target
HDEL
Clonality
Monoclonal
Clone
2E7
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G Purified
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.
Molecular Weight
78kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1 μg/ml was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Tested Applications
ICC, IF, WB
Host or Source
Mouse
Preservative
PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
Isotype
IgG2b

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