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HIF 1 alpha Antibody

CAT: 0013-GTR18316532Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18316532Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Anti-Mouse HIF-1 alpha Monoclonal IgG1
Product Name Alternative
ARNT interacting protein, HIF1A, Hypoxia inducible factor 1 alpha, MOP1, PASD8
UniProt
Q61221
Reactivity
Bovine, Human, Mouse, Rat
Immunogen
Mouse recombinant fragment corresponding to amino acids 329-530
Target
HIF 1 alpha
Clonality
Monoclonal
Clone
ESEE122
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction
Purification
Protein G Purified
Concentration
1 mg/ml
Dilution
WB (1:1000), IHC (1:100), ICC/IF (1:50) ; optimal dilutions for assays should be determined by the user.
Molecular Weight
116kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1 μg/ml was sufficient for detection of HIF1α in 20 μg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Tested Applications
ELISA, ICC, IF, IHC, WB
NCBI Accession Number
NP_034561.2
Host or Source
Mouse
Preservative
PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
Isotype
IgG1
Entrez
15251

UniProtKB · Q61221

Hypoxia-inducible factor 1-alpha

HIF1A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61221
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Hif1a
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
HIF-1-alpha, HIF1-alpha
EC number
—
Processing
—
Secondary accessions
O08741, O08993, Q61664, Q61665, Q8C681, Q8CC19, Q8CCB6, Q8R385, Q9CYA8
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmNucleus

Domain

Repeat

Biological process

TranscriptionTranscription regulation

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