Rabbit anti-ALG5 Antibody

• Background:

  Rabbit polyclonal antibody to ALG5

• Synonyms:

  Dolichyl-phosphate beta-glucosyltransferase; DolP-glucosyltransferase; Asparagine-linked glycosylation protein 5 homolog

• Host:

  Rabbit

• Reactivity:

  H

• Immunogen:

  Recombinant fusion protein of human ALG5. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of ALG5 expression in HepG2 (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of ALG5 staining in mouse pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of ALG5 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

• Gene ID (Human):

  29880

• Gene ID (Mouse):

  66248

• SwissProt (Human):

  Q9Y673

• SwissProt (Mouse):

  Q9DB25

• CAS Number:

  9007-83-4