Mouse anti-MBP Antibody

• Background:

  Mouse monoclonal antibody to MBP

• Synonyms:

  Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

• Host:

  Mouse

• Reactivity:

  H

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within human MBP. The exact sequence is proprietary.

• Clonality:

  Monoclonal

• Applications:

  IH

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  IH (1/100 - 1/300)

• Buffer:

  Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• IHC description:

  Immunohistochemical analysis of MBP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunohistochemical analysis of MBP staining in human cerebellum formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IP description:

  Immunohistochemical analysis of MBP staining in human hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  4155

• SwissProt (Human):

  P02686

• CAS Number:

  9007-83-4