Mouse anti-Villin Antibody

• Background:

  Mouse monoclonal antibody to Villin

• Synonyms:

  VIL; Villin-1

• Host:

  Mouse

• Reactivity:

  H

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within human Villin. The exact sequence is proprietary.

• Clonality:

  Monoclonal

• Applications:

  WB, IH

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/300)

• Buffer:

  Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of Villin expression in HepG2 (A), SW480 (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Villin staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunohistochemical analysis of Villin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  7429

• SwissProt (Human):

  P09327

• CAS Number:

  9007-83-4