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Mouse anti-PCNA Antibody

CAT: 0586-DL99572A-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0586-DL99572A-01Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mouse monoclonal antibody to PCNA
CAS Number
9007-83-4
Synonyms
Proliferating cell nuclear antigen; PCNA; Cyclin
Host
Mouse
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/300)
Buffer
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IHC description
Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunohistochemical analysis of PCNA staining in human squamous cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5111
SwissProt (Human)
P12004

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