Rabbit anti-CD83 Antibody

• Background:

  Rabbit polyclonal antibody to CD83

• Synonyms:

  CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD83

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD83. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of CD83 expression in rat thymus (A), mouse lung (B), HuT78 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of CD83 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  9308

• Gene ID (Mouse):

  12522

• SwissProt (Human):

  Q01151

• SwissProt (Mouse):

  O88324

• CAS Number:

  9007-83-4