Rabbit anti-KCHIP1 Antibody

• Background:

  Rabbit polyclonal antibody to KCHIP1

• Synonyms:

  KCHIP1; VABP; Kv channel-interacting protein 1; KChIP1; A-type potassium channel modulatory protein 1; Potassium channel-interacting protein 1; Vesicle APC-binding protein

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human KCHIP1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of KCHIP1 expression in CT26 (A), C6 (B), MCF7 (C), HEK293T (D), U87MG (E) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of KCHIP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of KCHIP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  30820

• Gene ID (Mouse):

  70357

• Gene ID (Rat):

  65023

• SwissProt (Human):

  Q9NZI2

• SwissProt (Mouse):

  Q9JJ57

• SwissProt (Rat):

  Q8R426

• CAS Number:

  9007-83-4