Rabbit anti-Kv2.1 Antibody

• Background:

  Rabbit polyclonal antibody to Kv2.1

• Synonyms:

  Potassium voltage-gated channel subfamily B member 1; Delayed rectifier potassium channel 1; DRK1; h-DRK1; Voltage-gated potassium channel subunit Kv2.1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kv2.1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of Kv2.1 expression in A375 (A), Panc1 (B), HCT116 (C), CT26 (D), C6 (E) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Kv2.1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of Kv2.1 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  3745

• Gene ID (Mouse):

  16500

• Gene ID (Rat):

  25736

• SwissProt (Human):

  Q14721

• SwissProt (Mouse):

  Q03717

• SwissProt (Rat):

  P15387

• CAS Number:

  9007-83-4