Rabbit anti-CD45 Antibody

• Background:

  Rabbit polyclonal antibody to CD45

• Synonyms:

  CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45

• Host:

  Rabbit

• Reactivity:

  H, M

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD45. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of CD45 expression in K562 (A), SHSY5Y (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of CD45 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of CD45 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  5788

• SwissProt (Human):

  P08575

• SwissProt (Mouse):

  P06800

• CAS Number:

  9007-83-4