Rabbit anti-HSF1 (pS121) Antibody

• Background:

  Rabbit polyclonal antibody to HSF1 (pS121)

• Synonyms:

  HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSF1 (pS121) . The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of HSF1 (pS121) expression in mouse lung (A), rat spleen (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of HSF1 (pS121) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  3297

• Gene ID (Mouse):

  15499

• SwissProt (Human):

  Q00613

• SwissProt (Mouse):

  P38532

• CAS Number:

  9007-83-4