Rabbit anti-ULK1 Antibody

• Background:

  Rabbit polyclonal antibody to ULK1

• Synonyms:

  KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ULK1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IH

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB Description:

  Western blot analysis of ULK1 expression in A549 (A), HEK293T (B), AML12 (C), C6 (D), Panc1 (E) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of ULK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  8408

• Gene ID (Mouse):

  22241

• SwissProt (Human):

  O75385

• SwissProt (Mouse):

  O70405

• CAS Number:

  9007-83-4