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Rabbit anti-CDC2 Antibody

CAT: 0586-DL90190A-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0586-DL90190A-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to CDC2
CAS Number
9007-83-4
Synonyms
CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
Host
Rabbit
Reactivity
H, M, R, B, D, Mk, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), FC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of E Cadherin expression in HEK293T (A), PC12 (B), A431 (C), MCF7 (D), C2C12 (E) whole cell lysates.
IHC description
Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
IP description
Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate, using 5ug of anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+) . No antibody was added to the control (-) . The antibody was incubated under agitation with Protein G beads for 10min, HEK293F whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-E Cadherin Antibody.
Gene ID (Human)
999; 1000; 1001; 1002
Gene ID (Rat)
83502; 83501
SwissProt (Human)
P12830; P19022; P22223; P55283
SwissProt (Rat)
Q9R0T4; Q9Z1Y3; Q63149

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