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Rabbit anti-DUSP6 Antibody

CAT: 0586-DL93390A-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0586-DL93390A-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to DUSP6
CAS Number
9007-83-4
Synonyms
MKP3; PYST1; Dual specificity protein phosphatase 6; Dual specificity protein phosphatase PYST1; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
Host
Rabbit
Reactivity
H, M, R, B, D, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DUSP6. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DUSP6 expression in HepG2 (A), NIH3T3 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of DUSP6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of DUSP6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
1848
Gene ID (Mouse)
67603
Gene ID (Rat)
116663
SwissProt (Human)
Q16828
SwissProt (Mouse)
Q9DBB1
SwissProt (Rat)
Q64346