Rabbit anti-FOXO1 Antibody

• Background:

  Rabbit polyclonal antibody to FOXO1

• Synonyms:

  FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma

• Host:

  Rabbit

• Reactivity:

  H, B, Mk, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IF/IC, ChIP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), ChIP (Use at an assay dependent concentration)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of FOXO1 expression in HeLa (A), MCF7 (B), Jurkat (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of FOXO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of FOXO1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  2308

• SwissProt (Human):

  Q12778

• CAS Number:

  9007-83-4