Rabbit anti-AKT Antibody

• Background:

  Rabbit polyclonal antibody to AKT

• Synonyms:

  PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, P, S

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of AKT expression in A549 (A), mouse brain (B), rat lung (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  207

• Gene ID (Mouse):

  11651

• Gene ID (Rat):

  24185

• SwissProt (Human):

  P31749

• SwissProt (Mouse):

  P31750

• SwissProt (Rat):

  P47196

• CAS Number:

  9007-83-4