Rabbit anti-GPT Antibody

• Background:

  Rabbit polyclonal antibody to GPT

• Synonyms:

  AAT1; GPT1; Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human GPT

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of GPT expression in mouse lung (A), rat liver (B), rat heart (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of GPT staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  2875

• Gene ID (Mouse):

  76282

• Gene ID (Rat):

  81670

• SwissProt (Human):

  P24298

• SwissProt (Mouse):

  Q8QZR5

• SwissProt (Rat):

  P25409

• CAS Number:

  9007-83-4