Rabbit anti-ATG5 Antibody

• Background:

  Rabbit polyclonal antibody to ATG5

• Synonyms:

  APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human ATG5

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of ATG5 expression in Hela (A), NIH3T3 (B), PC12 (C), rat bain (D) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of ATG5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  9474

• Gene ID (Mouse):

  11793

• SwissProt (Human):

  Q9H1Y0

• SwissProt (Mouse):

  Q99J83

• SwissProt (Rat):

  Q3MQ06

• CAS Number:

  9007-83-4