Rabbit anti-MKK4 (pS257) Antibody

• Background:

  Rabbit polyclonal antibody to MKK4 (pS257)

• Synonyms:

  JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK

• Host:

  Rabbit

• Reactivity:

  H, M

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK4. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of MKK4 (pS257) expression in K562 (A), Hela (B), MCF7 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of MKK4 (pS257) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  6416

• Gene ID (Mouse):

  26398

• SwissProt (Human):

  P45985

• SwissProt (Mouse):

  P47809

• CAS Number:

  9007-83-4