Rabbit anti-BOB1 Antibody

• Background :

  Rabbit polyclonal antibody to BOB1

• Synonyms :

  OBF1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1

• Host :

  Rabbit

• Reactivity :

  H

• Immunogen :

  Recombinant full length protein of human BOB1

• Clonality :

  Polyclonal

• Applications :

  WB, IF/IC

• Purification Method :

  The antibody was purified by immunogen affinity chromatography.

• Concentration :

  1mg/mL

• Dilution :

  WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

• Buffer :

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions :

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description :

  Western blot analysis of BOB1 expression in HT29 (A), mouse spleen (B), rat thymus (C) whole cell lysates.

• IF/ICC description :

  Immunofluorescent analysis of BOB1 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human) :

  5450

• SwissProt (Human) :

  Q16633

• CAS Number :

  9007-83-4