Mouse anti-Cleaved PARP1 Antibody

• Background:

  Mouse monoclonal antibody to Cleaved PARP1

• Synonyms:

  ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

• Host:

  Mouse

• Reactivity:

  H

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence of human Cleaved PARP1. The exact sequence is proprietary.

• Clonality:

  Monoclonal

• Applications:

  WB, IHC

• Concentration:

  1mg/mL

• Dilution:

  WB (1/2000 - 1/5000), IH (1/200 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Cleaved PARP1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  142

• SwissProt (Human):

  P09874

• CAS Number:

  9007-83-4