Recombinant Oncorhynchus mykiss Interleukin-10

Recombinant Oncorhynchus mykiss Interleukin-10

• UniProt: Q6L8N7
• Expression Region: 24-184aa
• Organism: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
• Target Sequence: RRVPCSDRCCSFVEGFPVRLKELRTAFSTIRDYYEANDELETSLLDEGILHHLKSPVGCHAMDSILKFYLDTVLPTAMNNRTQNNNDFKSPIDSIGNIFHELKKEIVQCRNYFSCKKPFDINEFISSYEKMQDKGLYKAMGELDLLFNYIEEYLVSKRRKH
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Cancer
• Assay Type: Developed Protein
• Relevance: Immune regulatory cytokine.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 26.3 kDa
• References & Citations: "Molecular cloning and expression analysis of rainbow trout (Oncorhynchus mykiss) interleukin-10 cDNAs." Inoue Y., Kamota S., Ito K., Yoshiura Y., Ototake M., Moritomo T., Nakanishi T. Fish Shellfish Immunol. 18:335-344 (2005)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.