SingleFlowEx CD62L PE-Cy1537

SingleFlowEx CD62L PE-Cy1537

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: DREG56
• Validated Applications: FC
• Concentration: 0.030 mg/mL
• Format: PE-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am J Respir Crit Care Med. 1999 Apr159(4 Pt 1):1205-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/10194167,*Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L-selectin signaling induced by sulfatides engagement. J Leukoc Biol. 2008 Oct84(4):1192-201., URL: https://www.ncbi.nlm.nih.gov/pubmed/18653462,*Killcock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin. J Biol Chem. 2009 Mar 27284(13):8833-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/19129194,*Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus. J Virol. 2010 Jul84(13):6527-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/20410263 ,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol. 2002 Aug 15169(4):1768-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/12165498,*Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A. 1990 Mar87(6):2244-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/2179952,*Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood. 1991 Aug 178(3):805-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/1713515,*Use of this product has not been published., URL: ,
• Other References: *Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14101(37):13519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/15353601?ordin,*Ding Z, Issekutz TB, Downey GP, Waddell TK: L-selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1101(11):4245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/12609846?ordin,*Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L-selectin (CD62L) cross-linking signals neutrophil adhesive functions via the Mac-1 (CD11b/CD18) beta 2-integrin. J Immunol. 1995 Aug 1155(3):1502-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/7543524?ordina,*von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood. 1993 Jul 182(1):182-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/7686786?ordina,*Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct263(4 Pt 2):H1034-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/1384360?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.