Human MHCE (Major Histocompatibility Complex Class I E) ELISA Kit

Human MHCE (Major Histocompatibility Complex Class I E) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCE in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E
• UniProt: P13747
• Field of Research: Signal transduction; Tumor immunity; Infection immunity; Immune molecule; Developmental science.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL