Fibronectin, rat

Fibronectin, rat

• Background: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Rat fibronectin 100%, rat collagen type I, II, III, IV and V <0.1%, rat elastin <0.1% (RIA at 1:5000 dilution).
• UniProt: P04937 (FINC_Rat)
• Host: Rabbit
• Applications: IHC (paraffin), IF, ELISA, Radioimmunoassay, WB
• Field of Research: Extracellular matrix
• Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile) Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0,5 mL of sterile water and store aliquots at -20°C For further dilution use appropriate antibody diluent, eg Art No PU00,2 for IHC
• Assay Principle: IHC(P), IFA, ELISA, RIA, IB (WB)
• Concentration: 1 mg/mL
• Form: Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile); Contains antimycotic substance, but; No BSA and sodium azide! Reconstitute with 0,5 mL of sterile water and store aliquots at -20°C; For further dilution use appropriate antibody diluent, e.g. Art; No; PU002 for IHC
• References & Citations: 1. Pankov R and Yamada KM (2002) Fibronectin at a glance. J Cell Sci. Oct 15;115(Pt 20):3861-3863. 2. Labat-Robert J (2002) Fibronectin in malignancy. Semin Cancer Biol. Jun;12(3):187-95.
• Storage Conditions: -20°C