HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]

HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]

• Description: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression.
• Product Name Alternative: 90 kda heat shock protein beta HSP90 beta antibody D6S182 antibody FLJ26984 antibody Heat shock 84 kDa antibody Heat shock 90kD protein 1, beta antibody Heat shock 90kDa protein 1 beta antibody Heat shock protein 90 alpha family class B member 1 antibody Heat shock protein 90 kDa antibody Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody Heat shock protein 90kDa alpha family class B member 1 antibody Heat shock protein beta antibody Heat shock protein HSP 90 beta antibody Heat shock protein HSP 90-beta antibody HS90B_HUMAN antibody HSP 84 antibody HSP 90 antibody HSP 90 b antibody HSP 90b antibody HSP84 antibody HSP90 BETA antibody hsp90ab1 antibody HSP90B antibody HSPC2 antibody HSPCB antibody
• Abbreviation: Heat shock protein HSP 90-beta HSP 90 Heat shock 84 kDa (HSP 84; HSP84) HSP90AB1 HSP90B HSPC2 HSPCB
• CAS Number: 9000-83-3
• Swiss Prot: P08238 Human, P11499 Mouse, P34058 Rat
• Cellular Locus: Cytoplasm
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human HSP90 Beta aa 311-360 / 724.
• Isotype: IgG1
• Clone: 2-1-G3-R
• Conjugation: Non-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell
• Positive Control: HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, HeLa.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000 ;IHC-P: 1:5,000 ;IF-Cell: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 83.3 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes