Mouse Lp-a (Lipoprotein a) ELISA Kit

Mouse Lp-a (Lipoprotein a) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: LP; AK38; APOA
• Field of Research: Metabolic pathway; Cardiovascular biology; Hepatology; Gastroenterology; Nutrition metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 µg/mL
• Sample Type: Serum, plasma and other biological fluids.
• Detection Range: 0.312-20 µg/mL
• Sensitivity: 0.128 µg/mL