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NSE/ENO2 Rabbit Polyclonal Antibody

CAT: 0013-GTR17690140Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17690140Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Anti-NSE/ENO2 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Product Name Alternative
Enolase 2 (gamma; neuronal) ; Gamma enolase; Neural enolase; Neuron specific enolase; NSE
UniProt
P09104
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NSE, which shares 95.1% and 100% amino acid (aa) sequence identity with mouse and rat NSE, respectively.
Target
Gamma-enolase
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction
Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Dilution
Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells
Form
Lyophilized
Molecular Weight
47 kDa
References & Citations
Valeria Manzini 1 2, Pamela Cappelletti 3, Nicola S Orefice 4 5 6, Ilaria Brentari 7, Michael J Rigby 4 5 8 9, Maria Lo Giudice 10, Marco Feligioni 3 11, Roberto Rivabene 1, Alessio Crestini 1, Francesco Manfredi 12, Giuseppina Talarico 13, Giuseppe Bruno 13, Massimo Corbo 3, Luigi Puglielli 4 5 14, Michela A Denti 15, Paola Piscopo miR-92a-3p and miR-320a are Upregulated in Plasma Neuron-Derived Extracellular Vesicles of Patients with Frontotemporal Dementia Mol Neurobiol, (2024)
Pubmed id
39138758
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
FC, ICC, IF, IHC, WB
Host or Source
Rabbit
Preservative
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Isotype
Rabbit IgG

UniProtKB · P09104

Gamma-enolase

ENOG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09104
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ENO2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
B7Z2X9, Q96J33
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmMembrane

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding

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