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CFIm68 Antibody / CPSF6

CAT: 0800-RQ7345Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7345Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cleavage and polyadenylation specificity factor subunit 6, also called Cleavage factor Im complex 68 kDa subunit (CFIm68), is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q16630
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Recombinant human protein (amino acids R50-Q176) was used as the immunogen for the CFIm68 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3F11E1
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CFIm68 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CFIm68 antibody is available for research use only.
Storage Conditions
After reconstitution, the CFIm68 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CFIm68 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE human colorectal adenocarcinoma tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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