XIAP Antibody

• Description:

  XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death. It is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P98170

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids A15-V244) was used as the immunogen for the XIAP antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  3G2G1

• Applications:

  WB

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the XIAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This XIAP antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the XIAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the XIAP antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) human HeLa, 2) human MCF7, 3) human PANC-1, 4) human U-87 MG, 5) rat heart, 6) rat testis, 7) mouse heart and 8) mouse testis tissue lysate with XIAP antibody. Expected molecular weight ~57 kDa.