Anti-HLA-C PE

Anti-HLA-C PE

• Background: HLA-C, a member of MHC class I glycoproteins, is one of polymorphysm typing targets, which are important for transplantation. The HLA system plays an important role in the occurrence and outcome of infectious diseases. The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population. It has been described that HLA-C interacts with human herpesvirus 8 MIR1 protein.
• Specifications: The mouse monoclonal antibody DT-9 recognizes an extracellular epitope on HLA-C member of MHC class I molecules. It does not crossreact with HLA-A or HLA-B allotypes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: Purified MHC class I molecules of tamarin origin
• Target Antigen: HLA-C
• Isotype: IgG2b
• Clone: DT-9
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Körner C, Simoneau CR, Schommers P, Granoff M, Ziegler M, Hölzemer A, Lunemann S, Chukwukelu J, Corleis B, Naranbhai V, Kwon DS, Scully EP, Jost S, Kirchhoff F, Carrington M, Altfeld M: HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe 2017 Jul 1222(1):111-119. , URL: https://pubmed.ncbi.nlm.nih.gov/28704647/,*Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains . Eur J Immunol. 2015 Aug45(8):2356-64., URL: https://pubmed.ncbi.nlm.nih.gov/25982269/,*Serena M, Parolini F, Biswas P, Sironi F, Blanco Miranda A, Zoratti E, Scupoli MT, Ziglio S, Valenzuela-Fernandez A, Gibellini D, Romanelli MG, Siccardi A, Malnati M, Beretta A, Zipeto D: HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity. Sci Rep. 2018 May 228:46991. doi: 10.1038/srep46991. , URL: https://pubmed.ncbi.nlm.nih.gov/29786690/,*Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, McMichael A, Brackenridge S: Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011 Apr85(7):3367-74. , URL: https://pubmed.ncbi.nlm.nih.gov/21248048/,*van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J: Modulation of human leukocyte antigen-C by human cytomegalovirus stimulates KIR2DS1 recognition by natural killer cells. Front Immunol. 2017 Mar 298:298. , URL: https://pubmed.ncbi.nlm.nih.gov/28424684/,*Parolini F, Biswas P, Serena M, Sironi F, Muraro V, Guizzardi E, Cazzoletti L, Scupoli MT, Gibellini D, Ugolotti E, Biassoni R, Beretta A, Malnati M, Romanelli MG, Zipeto D: Stability and expression levels of HLA-C on the cell membrane modulate HIV-1 infectivity. J Virol. 2017 Dec 1492(1):e01711-17. , URL: https://pubmed.ncbi.nlm.nih.gov/29070683/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.