Recombinant Guinea pig Cytotoxic T-lymphocyte associated protein 4 (CTLA4) , partial (Active)

• CAS Number:

  9000-83-3

• Gene Name:

  CTLA4

• UniProt:

  H0VUB1

• Expression Region:

  37-161aa

• Organism:

  Cavia porcellus

• Target Sequence:

  AMHVAQPAVVLASSRGVASFECEYASSHNANEVRVTVLQQVASRTTEICAATYTVERELAFPEDSACAGTSSGTRVNLTIQGLRAADTGLYICKVELMYPPPYFVGTGNGTQIYVIDPEPCPDSD

• Tag:

  C-terminal 6xHis-tagged

• Source:

  Yeast

• Field of Research:

  Immunology

• Assay Type:

  Active Protein & In Stock Protein

• Relevance:

  Cytotoxic Tlymphocyte 4 (CTLA-4, CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily.CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

• Endotoxin:

  Less than 1.0 EU/µg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-1-Fc at 5 μg/ml can bind Cavia porcellus CTLA-4-His, the ED50 of Recombinant Cavia porcellus CTLA-4-His is 4.29 ng/ml.

• Length:

  Partial

• Form:

  Liquid

• Buffer:

  0.2 μm filtered 1xPBS, pH 7.4

• Molecular Weight:

  14.1 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.