FITC-Labeled Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.

• Description:

  FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-HF2W4) is expressed from human 293 cells (HEK293) . It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) (Accession # P01732-1 (CD8A) & P10966-1 (CD8B) ) . It is the FITC labeled form of Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5) .

• Specifications:

  This protein . The protein has a calculated MW of 19.5 kDa & 22.1 kDa. The protein migrates as 30-32 kDa and 32-34 kDa under reducing (R) condition, and 30-33 kDa and 60-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P01732-1 & P10966-1

• Expression Region:

  Ser 22 - Asp 182 & Leu 22 - Pro 170

• Host:

  HEK293

• Target:

  CD8 alpha & beta

• Conjugation:

  FITC Labeled

• Tag:

  C-10xHis | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-HF2W4) with a linear range of 0.625-2.5 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  19.5 kDa & 22.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C, aviod light

• Package Size:

  200ug*1

• Species:

  Human