Anti-T7 Antibody
CAT:
14-18864-01-1
Size:
100 µg
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-T7 Antibody
- Description: Rabbit Polyclonal Antibody specific to T7
- Product Name Alternative: Anti-T7 Rabbit Polyclonal Antibody
- CAS Number: 9007-83-4
- Host: Rabbit
- Reactivity: T7
- Immunogen: T7 (MASMTGGQQMG) conjugated to KLH.
- Target: T7
- Clonality: Polyclonal
- Type: Antibody
- Applications: WB, IP, ICC, ELISA
- Field of Research: Epitope Tags
- Purification Method: Purified by peptide immuno-affinity chromatography
- Concentration: 1.0 mg/mL
- Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
- Format: Purified
- Form: Liquid
- Buffer: Phosphate Buffered Saline
- Additionnal Information: Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
Immunocytochemistry: use at a dilution of 1:100-1:400.
Immunoprecipitation: use 1-4ug/mg lysate.
ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500
These are recommended dilutions.
Endusers should determine optimal dilutions for their applications. - Storage Conditions: This antibody is stable for at least one (1) year at 2-8°C.
- Specificity: Antibody was affinity-purified on the peptide.
- Formulation: PBS, pH 7.2, 0.09% sodium azide.
- Buffer pH: pH 7.2
- Target Background: The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.