Endo S2/Endo-beta-N-acetylglucosaminidase

• Description:

  Endo S2 recognizes the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans.

• Product Name Alternative:

  Endo S2, Endo-S2, EndoS2, Endo-beta-N-acetylglucosaminidase

• Expression System:

  E.coli

• Nature:

  Recombinant

• Applications:

  Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.

• Stability:

  Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

• Endotoxin:

  Please contact with the lab for this information.

• Purity:

  >90% as determined by SDS-PAGE.

• Bioactivity:

  Unit Definition - One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from PBS pH7.4,1mM EDTA, 4%trehalose,1% mannitol.

• Reconstitution:

  Reconstitute in sterile water for a stock solution.

• Shipping Conditions:

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes:

  For research use only.

• Species:

  Streptococcus pyogenes