AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [JE75-09]

AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [JE75-09]

• Description: Akt, also referred to as PKB or Rac, plays a critical role in controlling cell survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 . Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.
• Product Name Alternative: AKT antibody AKT1 antibody AKT1 kinase antibody AKT1m antibody AKT2 antibody AKT2 kinase antibody Akt3 antibody AKT3_HUMAN antibody CAKT antibody CWS6 antibody DKFZp434N0250 antibody HIHGHH antibody kinase Akt1 antibody MGC99656 antibody MPPH antibody Murine thymoma viral (v-akt) homolog 2 antibody pan-akt antibody PKB ALPHA antibody PKB antibody PKB beta antibody PKB gamma antibody PKB-GAMMA antibody PKB/Akt antibody PKBALPHA antibody PKBB antibody PKBBETA antibody PKBG antibody PKBGAMMA antibody PRKBA antibody PRKBB antibody PRKBG antibody Protein kinase Akt 2 antibody Protein kinase Akt-2 antibody Protein kinase Akt-3 antibody Protein kinase B alpha antibody Protein kinase B antibody Protein kinase B beta antibody Protein kinase B gamma antibody Proto oncogene c Akt antibody Proto-oncogene c-Akt antibody RAC ALPHA antibody RAC alpha serine/threonine protein kinase antibody RAC antibody RAC BETA antibody RAC beta serine/threonine protein kinase antibody RAC PK alpha antibody RAC PK beta antibody rac protein kinase alpha antibody rac protein kinase beta antibody RAC-ALPHA antibody RAC-alpha serine/threonine-protein kinase antibody RAC-beta serine/threonine-protein kinase antibody RAC-gamma antibody RAC-gamma serine/threonine-protein kinase antibody RAC-PK-alpha antibody RAC-PK-beta antibody RAC-PK-gamma antibody RACALPHA antibody RACalpha serine/threonine kinase antibody RACBETA antibody RACgamma antibody RACgamma serine/threonine protein kinase antibody RACPKgamma antibody serine threonine protein kinase antibody STK 2 antibody STK-2 antibody STK2 antibody thymoma viral proto oncogene 1 antibody thymoma viral proto oncogene antibody V akt murine thymoma viral oncogene homolog 1 antibody V akt murine thymoma viral oncogene homolog 2 antibody V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody V akt murine thymoma viral oncogene homolog 3 antibody V-AKT murine thymoma viral oncogene homolog 1 antibody V-AKT murine thymoma viral oncogene homolog 2 antibody V-AKT murine thymoma viral oncogene homolog 3 antibody vakt murine thymoma viral oncogene homolog 1 antibody vakt murine thymoma viral oncogene homolog 2 antibody vakt murine thymoma viral oncogene homolog 3 antibody
• Abbreviation: AKT1, PKB, RAC, AKT2, AKT3, PKBG
• CAS Number: 9000-83-3
• Swiss Prot: P31749 Human, P31751 Human, Q9Y243 Human, P31750 Mouse, Q60823 Mouse, Q9WUA6 Mouse, P47196 Rat, P47197 Rat, Q63484 Rat
• Cellular Locus: Cytoplasm, Nucleus, Cell membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Monkey
• Immunogen: Recombinant protein within Human AKT1/2/3 aa 231-480 / 480.
• Isotype: IgG
• Clone: JE75-09
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, FC, IP
• Positive Control: HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, U-2 OS cell lysate, NIH/3T3 cell lysate, MCF7, COS-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, Rat testis tissue lysate, RAW264.7, C6.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 56 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes