S100A4 Antibody

• Description :

  S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

• Specifications :

  ELISA: order antibody without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P26447

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 1-200 from the human S100A4 protein was used as the immunogen for the S100A4 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  S100A4/6803

• Applications :

  ELISA, IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the S100A4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This S100A4 antibody is available for research use only.

• Storage Conditions :

  Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the S100A4 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasm, nucleus

• Image Legend :

  IHC staining of FFPE human colon tissue with S100A4 antibody (clone S100A4/6803) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.