MGMT Antibody

• Description:

  Cancer chemotherapeutic alkylating agents (e.g. BCNU, ) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P16455

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged MGMT protein was used as the immunogen for the MGMT antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  MGMT/4791

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MGMT antibody is available for research use only.

• Storage Conditions:

  Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MGMT antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human lymph node tissue with MGMT antibody (clone MGMT/4791) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.