EIF5 Antibody

CAT: 0800-RQ6953Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ6953Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P55010
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human EIF5 protein (amino acids D11-D423) was used as the immunogen for the EIF5 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
2F13C4
Applications
WB, IF, FACS
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the EIF5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This EIF5 antibody is available for research use only.
Storage Conditions
After reconstitution, the EIF5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the EIF5 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human PC-3 cells with EIF5 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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