EIF5 Antibody

• Description:

  Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P55010

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human EIF5 protein (amino acids D11-D423) was used as the immunogen for the EIF5 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  2F13C4

• Applications:

  WB, IF, FACS

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the EIF5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This EIF5 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the EIF5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the EIF5 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human PC-3 cells with EIF5 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.