Human beta Defensin-2 Recombinant

CAT: 0421-90107-BSize: 20 µgDry Ice: NoHazardous: No

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CAT#:0421-90107-BSize:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides. It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death. Unlike hBD-1, this second peptide, human beta-defensin-2 (hBD-2), is regulated at a transcriptional level in response to contact with microorganisms, and is highly effective in killing Gram negative bacteria. Its expression is also upregulated by the cytokine tumor necrosis factor-alpha.

Description

Recombinant beta Defensin-2 is a disulfide-linked monomer protein consisting of 42 amino acid residues, and migrates as an approximately 4 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-2 mature chain was expressed inE. coli.

UniProt

O15263

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 98% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

4 kDa

Formulation

Lyophilized from 0.2 µm filtered 100 mM NaCl, 20 mM phosphate buffer, pH 7.4.

UniProtKB · O15263

Defensin beta 4A

DFB4A_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: O15263
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: DEFB4A, DEFB4B
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: Q52LC0

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

AntibioticAntimicrobialDefensin

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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MBS232312-01	RECOMBINANT HUMAN DEFENSIN BETA 2
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MBS4159633-01	Recombinant Human Beta-Defensin 2 (DEFB4A)
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