Dicer, FLAG-Tag Recombinant

• Background:

  Dicer (also known as endoribonuclease Dicer or helicase with RNase motif) is a ribonuclease that participates in pre-microRNA (pre-miRNA) and double stranded RNA (dsRNA) cleavage. The cleavage of pre-miRNA into mature miRNA and formation of small interfering RNA (siRNA) from dsRNA are essential for gene regulation and DNA repair. Decreased levels of Dicer can result in Age-related Macular Degeneration (AMD), the most common age-related blindness. Dicer impairment is also involved in cancer and RNA-based viral infection. The development of small molecule inhibitors or other strategies that affect Dicer activity can provide new therapeutical avenues for cancer treatment and AMD prevention.

• Description:

  Recombinant human Dicer (Endoribonuclease Dicer), full length, encompassing amino acids 2-1922 (end) . This construct contains an N-terminal Flag-tag.  The recombinant protein was affinity purified.

• CAS Number:

  9000-83-3

• UniProt:

  Q9UPY3

• Tag:

  N-terminal FLAG-tag

• Purity:

  ≥ 85%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  220 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, and 20% glycerol