Protein Tyrosine Phosphatase-1B (PTP-1B) Activity Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Metabolic Diseases

• Assay Principle:

  Protein Tyrosine Phosphatase-1B (PTP-1B) belongs to the family of protein tyrosine phosphatases, which specifically hydrolyze aromatic phosphates and negatively regulate insulin signal transduction by dephosphorylating tyrosine residues on insulin receptors or their substrates. In the dephosphorylation of PTP-1B, the phosphate group produced reacts with malachite green to form chromogenic substance, PTP-1B in samples is measured by measuring the absorbance change at 636 nm.

• Assay Performance Time:

  1 h 50 min

• Sample Type:

  Animal and plant tissue; Cell

• Detection Range:

  0.216-7.200 U/L

• Sensitivity:

  0.216 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  7.5-10.0%

• Intra CV:

  2.0-2.1%

• Detection Instrument:

  Microplate reader