KRAS (G12V), Isoform B, His-Tag, BODIPY-GDP Loaded Recombinant

• Description:

  Recombinant human KRAS (GTPase), isoform b, encompassing amino acids 2-185 (end) . This construct contains an N-terminal His-tag (6xHis) mutation of interest G12V. KRAS (G12V) His-Tag (BPS Bioscience #100480) was affinity purified and was loaded with BODIPY-GDP. Unbound BODIPY-GDP was removed by spin column and the protein is ready for a GDP/GTP exchange assay.

• UniProt:

  P01116

• Label:

  BODIPY™ FL GDP ((ThermoFisher Scientific, Guanosine 5'-Diphosphate, BODIPY™ FL 2'- (or-3') -O- (N- (2-Aminoethyl) Urethane), Bis (Triethylammonium) Salt), is a mixed isomer in which the BODIPY® FL fluorophore has been attached to the 2’ or 3’ position of the ribose ring via a linker. Invitrogen BODIPY® FL dye is a green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor 488. It is characterized by a high extinction coefficient and high quantum yield and is relatively insensitive to pH changes. Its hydrophobic properties make it useful with lipophilic compounds. The dye has an excited-state lifetime of 5 nanoseconds or longer, making it useful for fluorescence polarization–based assays.

• Purity:

  ≥ 90%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C

• Calculated Molecular Weight:

  22 kDa

• Formulation:

  20 mM HEPES, pH 7.4,150 mM NaCl, and 1.0 mM DTT

• CAS Number:

  9000-83-3