ACE2 Lentivirus

• Description:

  Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found on the surface of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the two strains that caused outbreaks of Severe acute respiratory syndrome (SARS-CoV) and coronavirus disease 2019 (COVID-19) (SARS-CoV-2) .The ACE2 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an ACE2 gene (NM_021804.3) driven by an EF1A promoter (Figure 1) .Figure 1. Schematic of the lenti-vector used to generate the ACE2 lentivirusThis product has beencited 5 times.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

• Formulation:

  The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.