JMJD2D Homogeneous Assay Kit

CAT: 0421-79838Size: 384 ReactionsDry Ice: YesHazardous: No

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CAT#:0421-79838Size:384 Reactions

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Description

The JMJD2D Homogeneous Assay Kit is designed to measure JMJD2D activity for screening and profiling applications. JMJD2D, also known as KDM4D, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K9Me3and H3-K36Me3. The key to the JMJD2D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Scheme 1:Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.Need us to run inhibitor screens or profile your compounds against JMJD2D (KDM4D) ? Check out ourDemethylase Screening Services.

UniProt

Q6B0I6

Format

Catalog #ComponentAmountStorage50117JMJD2D (KDM4D) 1 µg-80°CAvoidfreeze/thawcycles!52140EPrimary antibody 520 µl-80°C79841Biotinylated histone H3 peptide substrate500 rxns-80°C524094x HDM Assay Buffer 43 ml-80°C523014x Detection buffer2 ml-20°C

Shipping Conditions

-80°C

Storage Conditions

At least 6 months from date of receipt when stored as directed.

Contraindications

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+and Ni2+) . The presence of > 1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

UniProtKB · Q6B0I6

Lysine-specific demethylase 4D

KDM4D_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q6B0I6
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: KDM4D
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 1.14.11.66
Processing: —
Secondary accessions: B3KPC4, Q0VF39, Q9NT41, Q9NW76

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

ADP-ribosylationUbl conjugation

Molecular function

Chromatin regulatorDioxygenaseOxidoreductase

Ligand

IronMetal-bindingZinc

Cellular component

Nucleus

Biological process

TranscriptionTranscription regulation

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